Glucose-6-phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase ofCandida tropicalisPartial Purification and Some Properties
نویسندگان
چکیده
منابع مشابه
Purification and properties of Penicillium glucose 6-phosphate dehydrogenase.
1. Glucose 6-phosphate dehydrogenase was isolated and partially purified from a thermophilic fungus, Penicillium duponti, and a mesophilic fungus, Penicillium notatum. 2. The molecular weight of the P. duponti enzyme was found to be 120000+/-10000 by gelfiltration and sucrose-density-gradient-centrifugation techniques. No NADP(+)- or glucose 6-phosphate-induced change in molecular weight could ...
متن کاملPurification and properties of d-glucose-6-phosphate dehydrogenase.
n-Glucose-6-phosphate dehydrogenase (Zwischenferment) was discovered by Warburg and Christian in 1931 (1, 2). An active preparation was obtained then from horse erythrocytes and in 1932 from the Lebedev juice made from brewers’ yeast (3). Because of its extreme specificity, this enzyme has become an important analytical tool. Various preparations of it have been described, the first being those...
متن کاملMicrofluorometry of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in red cells.
A simple microfluorometric method isdescribed for measu ring gIucose-6-phosphate dehyd rogenase (GPD) and 6-phosphogluconate dehydrogenase (PGD) activities in human erythrocytes. Only 50 ,J of whole blood is required. The cells are lysed with digitonin and the rate of NADPH generated in the enzymatic reactions is followed kinetically with a recording fluoromete. GPD activity is measured by subt...
متن کاملGlucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia
Background: Jaundice is affecting over 60-80 percent of neonates in the first week of life. Glucose-6-phosphate dehydrogenase (G6PD) deficiency, which is an important cause of pathologic hyperbilirubinemia, can lead to hemolytic anemia, jaundice and kernicterus. The present study was performed to determine the prevalence of G6PD deficiency among icteric neonates in Shirvan, Iran. Methods: This...
متن کاملFurther studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver.
Renewed interest in the direct oxidative pathway of glucose 6-phosphate metabolism during the last few years has revealed that this pathway is by no means restricted to erythrocytes, yeast and microorganisms. The triphosphopyridine-nucleotide(TPN)-specific glucose 6-phosphate and 6-phosphogluconate dehydrogenases are also widely distributed in mammalian tissues (Dickens & Glock, 1950, 1951; Hor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1976
ISSN: 0002-1369
DOI: 10.1080/00021369.1976.10862311